生物素在脂質、蛋白質和碳水化合物的代謝中發揮著關鍵作用。它是四種羧化酶的關鍵輔酶:乙酰輔酶A羧化酶(acetyl CoA carboxylase),那是涉及從醋酸鹽合成脂肪酸的過程;涉及糖異生(gluconeogenesis)的丙酮酸羧化酶(pyruvate carboxylase);涉及白胺酸代謝的β-甲基巴豆酰基輔酶A羧化酶(β-methylcrotonyl CoA carboxylase);與涉及能量、氨基酸和膽固醇代謝有關的丙酰輔酶A羧化酶(propionyl CoA carboxylase)[24]。
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