• regulation of transcription, DNA-templated • somatic stem cell population maintenance • negative regulation of gene silencing by miRNA • cell fate commitment involved in formation of primary germ layer • endodermal cell fate specification • anatomical structure morphogenesis • mRNA transcription by RNA polymerase II • regulation of asymmetric cell division • negative regulation of transcription by RNA polymerase II • transcription by RNA polymerase II • transcription, DNA-templated • regulation of DNA methylation-dependent heterochromatin assembly • multicellular organism development • cardiac cell fate determination • response to wounding • BMP signaling pathway involved in heart induction • 基因調節 • regulation of heart induction by regulation of canonical Wnt signaling pathway • blastocyst development • positive regulation of transcription by RNA polymerase II • positive regulation of SMAD protein signal transduction
^ 2.02.12.2GRCh38: Ensembl release 89: ENSG00000230336、ENSG00000206454、ENSG00000204531、ENSG00000237582、ENSG00000229094、ENSG00000233911、ENSG00000235068 - Ensembl, May 2017
^ 3.03.13.2GRCm38: Ensembl release 89: ENSMUSG00000024406 - Ensembl, May 2017
^Human PubMed Reference:. National Center for Biotechnology Information, U.S. National Library of Medicine.
^Mouse PubMed Reference:. National Center for Biotechnology Information, U.S. National Library of Medicine.
^Takeda J, Seino S, Bell GI. Human Oct3 gene family: cDNA sequences, alternative splicing, gene organization, chromosomal location, and expression at low levels in adult tissues. Nucleic Acids Research. Sep 1992, 20 (17): 4613–20. PMC 334192. PMID 1408763. doi:10.1093/nar/20.17.4613.
^Young Lab- Core Transcriptional Regulatory Circuitry in Human Embryonic Stem Cells （页面存档备份，存于互联网档案馆） at MIT
^Niwa H, Miyazaki J, Smith AG. Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells. Nature Genetics. Apr 2000, 24 (4): 372–6. PMID 10742100. doi:10.1038/74199.
^Petryniak B, Staudt LM, Postema CE, McCormack WT, Thompson CB. Characterization of chicken octamer-binding proteins demonstrates that POU domain-containing homeobox transcription factors have been highly conserved during vertebrate evolution. Proceedings of the National Academy of Sciences of the United States of America. Feb 1990, 87 (3): 1099–103. PMC 53418. PMID 1967834. doi:10.1073/pnas.87.3.1099.
Hough SR, Clements I, Welch PJ, Wiederholt KA. Differentiation of mouse embryonic stem cells after RNA interference-mediated silencing of OCT4 and Nanog. Stem Cells. Jun 2006, 24 (6): 1467–75. PMID 16456133. doi:10.1634/stemcells.2005-0475.
Feldman N, Gerson A, Fang J, Li E, Zhang Y, Shinkai Y, Cedar H, Bergman Y. G9a-mediated irreversible epigenetic inactivation of Oct-3/4 during early embryogenesis. Nature Cell Biology. Feb 2006, 8 (2): 188–94. PMID 16415856. doi:10.1038/ncb1353.
Boyer LA, Lee TI, Cole MF, Johnstone SE, Levine SS, Zucker JP, Guenther MG, Kumar RM, Murray HL, Jenner RG, Gifford DK, Melton DA, Jaenisch R, Young RA. Core transcriptional regulatory circuitry in human embryonic stem cells. Cell. Sep 2005, 122 (6): 947–56. PMC 3006442. PMID 16153702. doi:10.1016/j.cell.2005.08.020.
Gerrard L, Zhao D, Clark AJ, Cui W. Stably transfected human embryonic stem cell clones express OCT4-specific green fluorescent protein and maintain self-renewal and pluripotency. Stem Cells. 2005, 23 (1): 124–33. PMID 15625129. doi:10.1634/stemcells.2004-0102.
Reményi A, Lins K, Nissen LJ, Reinbold R, Schöler HR, Wilmanns M. Crystal structure of a POU/HMG/DNA ternary complex suggests differential assembly of Oct4 and Sox2 on two enhancers. Genes & Development. Aug 2003, 17 (16): 2048–59. PMC 196258. PMID 12923055. doi:10.1101/gad.269303.
Schoorlemmer J, Kruijer W. Octamer-dependent regulation of the kFGF gene in embryonal carcinoma and embryonic stem cells. Mechanisms of Development. Dec 1991, 36 (1-2): 75–86. PMID 1723621. doi:10.1016/0925-4773(91)90074-G.
Wey E, Lyons GE, Schäfer BW. A human POU domain gene, mPOU, is expressed in developing brain and specific adult tissues. European Journal of Biochemistry / FEBS. Mar 1994, 220 (3): 753–62. PMID 7908264. doi:10.1111/j.1432-1033.1994.tb18676.x.
Crouau-Roy B, Amadou C, Bouissou C, Clayton J, Vernet C, Ribouchon MT, Pontarotti P. Localization of the OTF3 gene within the human MHC class I region by physical and meiotic mapping. Genomics. May 1994, 21 (1): 241–3. PMID 8088794. doi:10.1006/geno.1994.1249.
Guillaudeux T, Mattei MG, Depetris D, Le Bouteiller P, Pontarotti P. In situ hybridization localizes the human OTF3 to chromosome 6p21.3-->p22 and OTF3L to 12p13. Cytogenetics and Cell Genetics. 1993, 63 (4): 212–4. PMID 8500351. doi:10.1159/000133537.
Abdel-Rahman B, Fiddler M, Rappolee D, Pergament E. Expression of transcription regulating genes in human preimplantation embryos. Human Reproduction. Oct 1995, 10 (10): 2787–92. PMID 8567814.
Hillier LD, Lennon G, Becker M, Bonaldo MF, Chiapelli B, Chissoe S, Dietrich N, DuBuque T, Favello A, Gish W, Hawkins M, Hultman M, Kucaba T, Lacy M, Le M, Le N, Mardis E, Moore B, Morris M, Parsons J, Prange C, Rifkin L, Rohlfing T, Schellenberg K, Bento Soares M, Tan F, Thierry-Meg J, Trevaskis E, Underwood K, Wohldman P, Waterston R, Wilson R, Marra M. Generation and analysis of 280,000 human expressed sequence tags. Genome Research. Sep 1996, 6 (9): 807–28. PMID 8889549. doi:10.1101/gr.6.9.807.
Inamoto S, Segil N, Pan ZQ, Kimura M, Roeder RG. The cyclin-dependent kinase-activating kinase (CAK) assembly factor, MAT1, targets and enhances CAK activity on the POU domains of octamer transcription factors. The Journal of Biological Chemistry. Nov 1997, 272 (47): 29852–8. PMID 9368058. doi:10.1074/jbc.272.47.29852.
Nichols J, Zevnik B, Anastassiadis K, Niwa H, Klewe-Nebenius D, Chambers I, Schöler H, Smith A. Formation of pluripotent stem cells in the mammalian embryo depends on the POU transcription factor Oct4. Cell. Oct 1998, 95 (3): 379–91. PMID 9814708. doi:10.1016/S0092-8674(00)81769-9.
Gonzalez MI, Robins DM. Oct-1 preferentially interacts with androgen receptor in a DNA-dependent manner that facilitates recruitment of SRC-1. The Journal of Biological Chemistry. Mar 2001, 276 (9): 6420–8. PMID 11096094. doi:10.1074/jbc.M008689200.
Butteroni C, De Felici M, Schöler HR, Pesce M. Phage display screening reveals an association between germline-specific transcription factor Oct-4 and multiple cellular proteins. Journal of Molecular Biology. Dec 2000, 304 (4): 529–40. PMID 11099378. doi:10.1006/jmbi.2000.4238.
Ezashi T, Ghosh D, Roberts RM. Repression of Ets-2-induced transactivation of the tau interferon promoter by Oct-4. Molecular and Cellular Biology. Dec 2001, 21 (23): 7883–91. PMC 99954. PMID 11689681. doi:10.1128/MCB.21.23.7883-7891.2001.
Guo Y, Costa R, Ramsey H, Starnes T, Vance G, Robertson K, Kelley M, Reinbold R, Scholer H, Hromas R. The embryonic stem cell transcription factors Oct-4 and FoxD3 interact to regulate endodermal-specific promoter expression. Proceedings of the National Academy of Sciences of the United States of America. Mar 2002, 99 (6): 3663–7. PMC 122580. PMID 11891324. doi:10.1073/pnas.062041099.
Looijenga LH, Stoop H, de Leeuw HP, de Gouveia Brazao CA, Gillis AJ, van Roozendaal KE, van Zoelen EJ, Weber RF, Wolffenbuttel KP, van Dekken H, Honecker F, Bokemeyer C, Perlman EJ, Schneider DT, Kononen J, Sauter G, Oosterhuis JW. POU5F1 (OCT3/4) identifies cells with pluripotent potential in human germ cell tumors. Cancer Research. May 2003, 63 (9): 2244–50. PMID 12727846.
Wang P, Branch DR, Bali M, Schultz GA, Goss PE, Jin T. The POU homeodomain protein OCT3 as a potential transcriptional activator for fibroblast growth factor-4 (FGF-4) in human breast cancer cells. The Biochemical Journal. Oct 2003, 375 (Pt 1): 199–205. PMC 1223663. PMID 12841847. doi:10.1042/BJ20030579.
Reményi A, Lins K, Nissen LJ, Reinbold R, Schöler HR, Wilmanns M. Crystal structure of a POU/HMG/DNA ternary complex suggests differential assembly of Oct4 and Sox2 on two enhancers. Genes & Development. Aug 2003, 17 (16): 2048–59. PMC 196258. PMID 12923055. doi:10.1101/gad.269303.
Rajpert-De Meyts E, Hanstein R, Jørgensen N, Graem N, Vogt PH, Skakkebaek NE. Developmental expression of POU5F1 (OCT-3/4) in normal and dysgenetic human gonads. Human Reproduction. Jun 2004, 19 (6): 1338–44. PMID 15105401. doi:10.1093/humrep/deh265.
Matin MM, Walsh JR, Gokhale PJ, Draper JS, Bahrami AR, Morton I, Moore HD, Andrews PW. Specific knockdown of Oct4 and beta2-microglobulin expression by RNA interference in human embryonic stem cells and embryonic carcinoma cells. Stem Cells. 2005, 22 (5): 659–68. PMID 15342930. doi:10.1634/stemcells.22-5-659.
Baal N, Reisinger K, Jahr H, Bohle RM, Liang O, Münstedt K, Rao CV, Preissner KT, Zygmunt MT. Expression of transcription factor Oct-4 and other embryonic genes in CD133 positive cells from human umbilical cord blood. Thrombosis and Haemostasis. Oct 2004, 92 (4): 767–75. PMID 15467907. doi:10.1160/TH04-02-0079.
Generating iPS Cells from MEFS through Forced Expression of Sox-2, Oct-4, c-Myc, and Klf4（页面存档备份，存于互联网档案馆）
十月 18, 2023
oct4, 八聚體結合轉錄因子4, octamer, binding, transcription, factor, 亦稱爲pou5f1, 第五類pou結構域轉錄因子1, 是一種蛋白質, 在人體中由pou5f1基因編碼, 是一種具有與pou結構域, 英语, domain, 同源的結構域的轉錄因子, 對未分化的胚胎幹細胞的自我更新與幹性維持至關重要, 也正因如此, 有時會被用來作標記未分化細胞的分子標記, 的過表達或低表達都會造成細胞的分化, 因此只在特定的表達強度下才能維持細胞的幹性, 已知的結構pdb直系同源搜索. Oct4 八聚體結合轉錄因子4 octamer binding transcription factor 4 亦稱爲POU5F1 第五類POU結構域轉錄因子1 是一種蛋白質 在人體中由POU5F1基因編碼 6 Oct4是一種具有與POU結構域 英语 POU domain 同源的結構域的轉錄因子 Oct4對未分化的胚胎幹細胞的自我更新與幹性維持至關重要 7 也正因如此 有時Oct4會被用來作標記未分化細胞的分子標記 Oct4的過表達或低表達都會造成細胞的分化 因此Oct4只在特定的表達強度下才能維持細胞的幹性 8 Oct4已知的結構PDB直系同源搜索 PDBe RCSBPDBID列表1OCP 3L1P識別號别名POU5F1 OCT3 OCT4 OTF 3 OTF3 OTF4 Oct 3 Oct 4 POU class 5 homeobox 1 Oct3 4外部IDOMIM 164177 MGI 101893 HomoloGene 8422 GeneCards POU5F1相關疾病史蒂芬斯 強森症候群 1 基因位置 人类 染色体6號染色體 2 基因座6p21 33起始31 164 337 bp 2 终止31 180 731 bp 2 基因位置 小鼠 染色体小鼠17号染色体 3 基因座17 B1 17 18 69 cM起始35 816 915 bp 3 终止35 821 669 bp 3 RNA表达模式查阅更多表达数据基因本體分子功能 DNA结合 sequence specific DNA binding miRNA binding DNA结合转录因子活性 转录因子结合 transcription cis regulatory region binding 血浆蛋白结合 DNA结合转录阻遏物活性 RNA聚合酶II特异性 DNA binding transcription factor activity RNA polymerase II specific 泛素蛋白连接酶结合 RNA binding細胞組分 細胞質 细胞质基质 轉錄調節複合物 核质 细胞核 線粒體生物學過程 regulation of transcription DNA templated somatic stem cell population maintenance negative regulation of gene silencing by miRNA cell fate commitment involved in formation of primary germ layer endodermal cell fate specification anatomical structure morphogenesis mRNA transcription by RNA polymerase II regulation of asymmetric cell division negative regulation of transcription by RNA polymerase II transcription by RNA polymerase II transcription DNA templated regulation of DNA methylation dependent heterochromatin assembly multicellular organism development cardiac cell fate determination response to wounding BMP signaling pathway involved in heart induction 基因調節 regulation of heart induction by regulation of canonical Wnt signaling pathway blastocyst development positive regulation of transcription by RNA polymerase II positive regulation of SMAD protein signal transductionSources Amigo QuickGO直系同源物種人類小鼠Entrez546018999EnsemblENSG00000230336 ENSG00000206454 ENSG00000204531 ENSG00000237582 ENSG00000229094 ENSG00000233911 ENSG00000235068ENSMUSG00000024406UniProtQ01860P20263mRNA 序列NM 203289 NM 001173531 NM 001285986 NM 001285987 NM 002701NM 001252452 NM 013633蛋白序列NP 001167002 NP 001272915 NP 001272916 NP 002692 NP 976034NP 001272915 1NP 001239381 NP 038661基因位置 UCSC Chr 6 31 16 31 18 MbChr 17 35 82 35 82 MbPubMed 查找 4 5 維基數據檢視 編輯人類檢視 編輯小鼠Oct4 八聚體結合轉錄因子4 名稱中的 八聚體 octamer 指DNA核苷酸序列 ATTTGCAT 9 目录 1 參見 2 參考 3 拓展閱讀 4 外部連結參見 编辑Sox2 Nanog參考 编辑 與Oct4相關的疾病 在維基數據上查看 編輯參考 2 0 2 1 2 2 GRCh38 Ensembl release 89 ENSG00000230336 ENSG00000206454 ENSG00000204531 ENSG00000237582 ENSG00000229094 ENSG00000233911 ENSG00000235068 Ensembl May 2017 3 0 3 1 3 2 GRCm38 Ensembl release 89 ENSMUSG00000024406 Ensembl May 2017 Human PubMed Reference National Center for Biotechnology Information U S National Library of Medicine Mouse PubMed Reference National Center for Biotechnology Information U S National Library of Medicine Takeda J Seino S Bell GI Human Oct3 gene family cDNA sequences alternative splicing gene organization chromosomal location and expression at low levels in adult tissues Nucleic Acids Research Sep 1992 20 17 4613 20 PMC 334192 nbsp PMID 1408763 doi 10 1093 nar 20 17 4613 Young Lab Core Transcriptional Regulatory Circuitry in Human Embryonic Stem Cells 页面存档备份 存于互联网档案馆 at MIT Niwa H Miyazaki J Smith AG Quantitative expression of Oct 3 4 defines differentiation dedifferentiation or self renewal of ES cells Nature Genetics Apr 2000 24 4 372 6 PMID 10742100 doi 10 1038 74199 Petryniak B Staudt LM Postema CE McCormack WT Thompson CB Characterization of chicken octamer binding proteins demonstrates that POU domain containing homeobox transcription factors have been highly conserved during vertebrate evolution Proceedings of the National Academy of Sciences of the United States of America Feb 1990 87 3 1099 103 PMC 53418 nbsp PMID 1967834 doi 10 1073 pnas 87 3 1099 拓展閱讀 编辑Lamoury FM Croitoru Lamoury J Brew BJ Undifferentiated mouse mesenchymal stem cells spontaneously express neural and stem cell markers Oct 4 and Rex 1 Cytotherapy 2006 8 3 228 42 PMID 16793732 doi 10 1080 14653240600735875 Hough SR Clements I Welch PJ Wiederholt KA Differentiation of mouse embryonic stem cells after RNA interference mediated silencing of OCT4 and Nanog Stem Cells Jun 2006 24 6 1467 75 PMID 16456133 doi 10 1634 stemcells 2005 0475 Feldman N Gerson A Fang J Li E Zhang Y Shinkai Y Cedar H Bergman Y G9a mediated irreversible epigenetic inactivation of Oct 3 4 during early embryogenesis Nature Cell Biology Feb 2006 8 2 188 94 PMID 16415856 doi 10 1038 ncb1353 Boyer LA Lee TI Cole MF Johnstone SE Levine SS Zucker JP Guenther MG Kumar RM Murray HL Jenner RG Gifford DK Melton DA Jaenisch R Young RA Core transcriptional regulatory circuitry in human embryonic stem cells Cell Sep 2005 122 6 947 56 PMC 3006442 nbsp PMID 16153702 doi 10 1016 j cell 2005 08 020 Gerrard L Zhao D Clark AJ Cui W Stably transfected human embryonic stem cell clones express OCT4 specific green fluorescent protein and maintain self renewal and pluripotency Stem Cells 2005 23 1 124 33 PMID 15625129 doi 10 1634 stemcells 2004 0102 Remenyi A Lins K Nissen LJ Reinbold R Scholer HR Wilmanns M Crystal structure of a POU HMG DNA ternary complex suggests differential assembly of Oct4 and Sox2 on two enhancers Genes amp Development Aug 2003 17 16 2048 59 PMC 196258 nbsp PMID 12923055 doi 10 1101 gad 269303 Schoorlemmer J Kruijer W Octamer dependent regulation of the kFGF gene in embryonal carcinoma and embryonic stem cells Mechanisms of Development Dec 1991 36 1 2 75 86 PMID 1723621 doi 10 1016 0925 4773 91 90074 G Wey E Lyons GE Schafer BW A human POU domain gene mPOU is expressed in developing brain and specific adult tissues European Journal of Biochemistry FEBS Mar 1994 220 3 753 62 PMID 7908264 doi 10 1111 j 1432 1033 1994 tb18676 x Crouau Roy B Amadou C Bouissou C Clayton J Vernet C Ribouchon MT Pontarotti P Localization of the OTF3 gene within the human MHC class I region by physical and meiotic mapping Genomics May 1994 21 1 241 3 PMID 8088794 doi 10 1006 geno 1994 1249 Guillaudeux T Mattei MG Depetris D Le Bouteiller P Pontarotti P In situ hybridization localizes the human OTF3 to chromosome 6p21 3 gt p22 and OTF3L to 12p13 Cytogenetics and Cell Genetics 1993 63 4 212 4 PMID 8500351 doi 10 1159 000133537 Abdel Rahman B Fiddler M Rappolee D Pergament E Expression of transcription regulating genes in human preimplantation embryos Human Reproduction Oct 1995 10 10 2787 92 PMID 8567814 Hillier LD Lennon G Becker M Bonaldo MF Chiapelli B Chissoe S Dietrich N DuBuque T Favello A Gish W Hawkins M Hultman M Kucaba T Lacy M Le M Le N Mardis E Moore B Morris M Parsons J Prange C Rifkin L Rohlfing T Schellenberg K Bento Soares M Tan F Thierry Meg J Trevaskis E Underwood K Wohldman P Waterston R Wilson R Marra M Generation and analysis of 280 000 human expressed sequence tags Genome Research Sep 1996 6 9 807 28 PMID 8889549 doi 10 1101 gr 6 9 807 Inamoto S Segil N Pan ZQ Kimura M Roeder RG The cyclin dependent kinase activating kinase CAK assembly factor MAT1 targets and enhances CAK activity on the POU domains of octamer transcription factors The Journal of Biological Chemistry Nov 1997 272 47 29852 8 PMID 9368058 doi 10 1074 jbc 272 47 29852 Nichols J Zevnik B Anastassiadis K Niwa H Klewe Nebenius D Chambers I Scholer H Smith A Formation of pluripotent stem cells in the mammalian embryo depends on the POU transcription factor Oct4 Cell Oct 1998 95 3 379 91 PMID 9814708 doi 10 1016 S0092 8674 00 81769 9 Gonzalez MI Robins DM Oct 1 preferentially interacts with androgen receptor in a DNA dependent manner that facilitates recruitment of SRC 1 The Journal of Biological Chemistry Mar 2001 276 9 6420 8 PMID 11096094 doi 10 1074 jbc M008689200 Butteroni C De Felici M Scholer HR Pesce M Phage display screening reveals an association between germline specific transcription factor Oct 4 and multiple cellular proteins Journal of Molecular Biology Dec 2000 304 4 529 40 PMID 11099378 doi 10 1006 jmbi 2000 4238 Ezashi T Ghosh D Roberts RM Repression of Ets 2 induced transactivation of the tau interferon promoter by Oct 4 Molecular and Cellular Biology Dec 2001 21 23 7883 91 PMC 99954 nbsp PMID 11689681 doi 10 1128 MCB 21 23 7883 7891 2001 Guo Y Costa R Ramsey H Starnes T Vance G Robertson K Kelley M Reinbold R Scholer H Hromas R The embryonic stem cell transcription factors Oct 4 and FoxD3 interact to regulate endodermal specific promoter expression Proceedings of the National Academy of Sciences of the United States of America Mar 2002 99 6 3663 7 PMC 122580 nbsp PMID 11891324 doi 10 1073 pnas 062041099 Looijenga LH Stoop H de Leeuw HP de Gouveia Brazao CA Gillis AJ van Roozendaal KE van Zoelen EJ Weber RF Wolffenbuttel KP van Dekken H Honecker F Bokemeyer C Perlman EJ Schneider DT Kononen J Sauter G Oosterhuis JW POU5F1 OCT3 4 identifies cells with pluripotent potential in human germ cell tumors Cancer Research May 2003 63 9 2244 50 PMID 12727846 Wang P Branch DR Bali M Schultz GA Goss PE Jin T The POU homeodomain protein OCT3 as a potential transcriptional activator for fibroblast growth factor 4 FGF 4 in human breast cancer cells The Biochemical Journal Oct 2003 375 Pt 1 199 205 PMC 1223663 nbsp PMID 12841847 doi 10 1042 BJ20030579 Remenyi A Lins K Nissen LJ Reinbold R Scholer HR Wilmanns M Crystal structure of a POU HMG DNA ternary complex suggests differential assembly of Oct4 and Sox2 on two enhancers Genes amp Development Aug 2003 17 16 2048 59 PMC 196258 nbsp PMID 12923055 doi 10 1101 gad 269303 Rajpert De Meyts E Hanstein R Jorgensen N Graem N Vogt PH Skakkebaek NE Developmental expression of POU5F1 OCT 3 4 in normal and dysgenetic human gonads Human Reproduction Jun 2004 19 6 1338 44 PMID 15105401 doi 10 1093 humrep deh265 Matin MM Walsh JR Gokhale PJ Draper JS Bahrami AR Morton I Moore HD Andrews PW Specific knockdown of Oct4 and beta2 microglobulin expression by RNA interference in human embryonic stem cells and embryonic carcinoma cells Stem Cells 2005 22 5 659 68 PMID 15342930 doi 10 1634 stemcells 22 5 659 Baal N Reisinger K Jahr H Bohle RM Liang O Munstedt K Rao CV Preissner KT Zygmunt MT Expression of transcription factor Oct 4 and other embryonic genes in CD133 positive cells from human umbilical cord blood Thrombosis and Haemostasis Oct 2004 92 4 767 75 PMID 15467907 doi 10 1160 TH04 02 0079 外部連結 编辑醫學主題詞表 MeSH Oct 4 Transcription Factor FactorBook POU5F1 Generating iPS Cells from MEFS through Forced Expression of Sox 2 Oct 4 c Myc and Klf4 页面存档备份 存于互联网档案馆 取自 https zh wikipedia org w index php title Oct4 amp oldid 79406536, 维基百科，wiki，书籍，书籍，图书馆，
