• neuron projection morphogenesis • RNA processing • negative regulation of transcription by RNA polymerase II • peripheral nervous system myelin formation • pre-miRNA processing • positive regulation of myelination • nerve development • RNA phosphodiester bond hydrolysis, endonucleolytic • positive regulation of Schwann cell differentiation • negative regulation of Schwann cell proliferation • production of miRNAs involved in gene silencing by miRNA • miRNA metabolic process • production of siRNA involved in RNA interference • 基因沉默 • apoptotic DNA fragmentation • 腫瘤壞死因子產生的負調控 • NIK/NF-kappaB signaling • negative regulation of gene expression • tube formation
^ 2.02.12.2GRCh38: Ensembl release 89: ENSG00000100697 - Ensembl, May 2017
^ 3.03.13.2GRCm38: Ensembl release 89: ENSMUSG00000041415 - Ensembl, May 2017
^Human PubMed Reference:. National Center for Biotechnology Information, U.S. National Library of Medicine.
^Mouse PubMed Reference:. National Center for Biotechnology Information, U.S. National Library of Medicine.
^Bernstein E, Caudy A, Hammond S, Hannon G. Role for a bidentate ribonuclease in the initiation step of RNA interference. Nature. 2001, 409 (6818): 363–6. PMID 11201747. doi:10.1038/35053110.
^ 7.07.1Macrae IJ, Zhou K, Li F, Repic A, Brooks AN, Cande WZ, Adams PD, Doudna JA. Structural basis for double-stranded RNA processing by Dicer. Science. Jan 2006, 311 (5758): 195–8. PMID 16410517. doi:10.1126/science.1121638.
^Matsuda S, Ichigotani Y, Okuda T, Irimura T, Nakatsugawa S, Hamaguchi M. Molecular cloning and characterization of a novel human gene (HERNA) which encodes a putative RNA-helicase. Biochimica et Biophysica Acta. Jan 2000, 1490 (1-2): 163–9. PMID 10786632. doi:10.1016/S0167-4781(99)00221-3.
^Hammond SM. Dicing and slicing: the core machinery of the RNA interference pathway. FEBS Letters. Oct 2005, 579 (26): 5822–9. PMID 16214139. doi:10.1016/j.febslet.2005.08.079.
^ 11.011.1Cenik ES, Fukunaga R, Lu G, Dutcher R, Wang Y, Tanaka Hall TM, Zamore PD. Phosphate and R2D2 restrict the substrate specificity of Dicer-2, an ATP-driven ribonuclease. Molecular Cell. Apr 2011, 42 (2): 172–84. PMC 3115569. PMID 21419681. doi:10.1016/j.molcel.2011.03.002.
四月 07, 2024
dicer, 蛋白在人體內由dicer1基因編碼, 是一種在rna干擾中扮演着重要角色的rna酶, 屬於iii型rna酶, 在rna誘導沉默複合體, risc, 的激活中, 扮演着核心角色, 在rna干擾過程中, 可以將shrna, 小髮卡rna, 切割, 加工爲sirna, 小干擾rna, 將mirna前體加工爲mirna, 微rna, dicer酶已知的結構pdb直系同源搜索, pdbe, rcsbpdbid列表2eb1, 4ngb, 4ngc, 4ngd, 4ngf, 4ngg, 4nh3, 4nh5, 4n. Dicer蛋白在人體內由DICER1基因編碼 是一種在RNA干擾中扮演着重要角色的RNA酶 屬於III型RNA酶 在RNA誘導沉默複合體 RISC 的激活中 Dicer扮演着核心角色 在RNA干擾過程中 Dicer可以將shRNA 小髮卡RNA 切割 加工爲siRNA 小干擾RNA 將miRNA前體加工爲miRNA 微RNA DICER酶已知的結構PDB直系同源搜索 PDBe RCSBPDBID列表2EB1 4NGB 4NGC 4NGD 4NGF 4NGG 4NH3 4NH5 4NH6 4NHA 4WYQ識別號别名DICER1 DCR1 Dicer Dicer1e HERNA MNG1 RMSE2 K12H4 8 LIKE dicer 1 ribonuclease III GLOW外部IDOMIM 606241 MGI 2177178 HomoloGene 13251 GeneCards DICER1相關疾病胸膜肺母細胞瘤 胚胎性横纹肌肉瘤 DICER1 syndrome familial multinodular goiter 1 基因位置 人类 染色体14號染色體 2 基因座14q32 13起始95 086 228 bp 2 终止95 158 010 bp 2 基因位置 小鼠 染色体小鼠12号染色体 3 基因座12 E 12 54 83 cM起始104 654 001 bp 3 终止104 718 211 bp 3 基因本體分子功能 核苷酸結合 helicase activity protein domain specific binding pre miRNA binding 金屬離子結合 endoribonuclease activity endoribonuclease activity producing 5 phosphomonoesters 血浆蛋白结合 siRNA binding RNA binding nuclease activity double stranded RNA binding endonuclease activity 水解酶活性 ATP結合 ribonuclease III activity DNA结合 deoxyribonuclease I activity細胞組分 細胞質 细胞质基质 RNA誘導沉默複合體 生长锥 ARC complex RISC loading complex 轴突 树突 细胞核 endoplasmic reticulum Golgi intermediate compartment 外排體生物學過程 neuron projection morphogenesis RNA processing negative regulation of transcription by RNA polymerase II peripheral nervous system myelin formation pre miRNA processing positive regulation of myelination nerve development RNA phosphodiester bond hydrolysis endonucleolytic positive regulation of Schwann cell differentiation negative regulation of Schwann cell proliferation production of miRNAs involved in gene silencing by miRNA miRNA metabolic process production of siRNA involved in RNA interference 基因沉默 apoptotic DNA fragmentation 腫瘤壞死因子產生的負調控 NIK NF kappaB signaling negative regulation of gene expression tube formationSources Amigo QuickGO直系同源物種人類小鼠Entrez23405192119EnsemblENSG00000100697ENSMUSG00000041415UniProtQ9UPY3Q8R418 F8VQ54mRNA 序列 NM 001195573 NM 001271282 NM 001291628 NM 030621 NM 177438NM 148948蛋白序列NP 001182502 NP 001258211 NP 001278557 NP 085124 NP 803187NP 683750基因位置 UCSC Chr 14 95 09 95 16 MbChr 12 104 65 104 72 MbPubMed 查找 4 5 維基數據檢視 編輯人類檢視 編輯小鼠 目录 1 功能 2 結構 3 參見 4 參考功能 编辑 nbsp Dicer酶的作用是將shRNA剪切爲siRNA或將miRNA前體剪切爲miRNADicer酶會將轉錄而成的 經Drosha酶初步處理的shRNA 小髮卡RNA 剪切爲siRNA 或將miRNA前體 pre miRNA 剪切爲miRNA 隨後 siRNA或miRNA會激活RISC的組裝和功能的行使 使RNA干擾得以繼續進行 6 結構 编辑 nbsp 肠贾第鞭毛虫 Giardia intestinalis 體內的Dicer蛋白 III型RNA酶結構域以綠色標出 PAZ以黃色標出 平臺結構域以紅色標出 連接不同結構域的螺旋以藍色標出 7 人DICER蛋白屬於III型RNA酶 具有解旋酶活性以及PAZ結構域 Piwi 英语 Piwi Argonaute Zwille 8 9 除了上述結構域外 DICER蛋白還有兩個RNaseIII結構域和兩個雙鏈RNA結合結構域 DUF283和dsRBD 10 目前的研究表明PAZ結構域可以和shRNA 小髮卡RNA 3 端兩個凸出的核苷酸結合 III型RNA酶結構域則會在dsRNA周圍形成一個假二聚體 啓動核酸鏈的剪切 使shRNA長度逐漸縮短 PAZ結構域和III型RNA酶結構域之間的距離由連接他們的螺旋結構決定 會影響到產生的miRNA siRNA長度 7 Dicer的dsRBD結構域會與shRNA結合 不過目前並未解析出具體的結合位點 推測認爲dsRBD區可能會與一些調節蛋白 比如人體內的TRBP 果蠅體內的R2D2 Loqs 結合形成複合體 調控RNA酶活性 進而調節終產物的產生 11 有推測認爲解旋酶結構域會參與對長底物的處理 11 參見 编辑RNA干擾 RNA誘導沉默複合體參考 编辑 與DICER酶相關的疾病 在維基數據上查看 編輯參考 2 0 2 1 2 2 GRCh38 Ensembl release 89 ENSG00000100697 Ensembl May 2017 3 0 3 1 3 2 GRCm38 Ensembl release 89 ENSMUSG00000041415 Ensembl May 2017 Human PubMed Reference National Center for Biotechnology Information U S National Library of Medicine Mouse PubMed Reference National Center for Biotechnology Information U S National Library of Medicine Bernstein E Caudy A Hammond S Hannon G Role for a bidentate ribonuclease in the initiation step of RNA interference Nature 2001 409 6818 363 6 PMID 11201747 doi 10 1038 35053110 7 0 7 1 Macrae IJ Zhou K Li F Repic A Brooks AN Cande WZ Adams PD Doudna JA Structural basis for double stranded RNA processing by Dicer Science Jan 2006 311 5758 195 8 PMID 16410517 doi 10 1126 science 1121638 Entrez Gene DICER1 Dicer1 Dcr 1 homolog Drosophila 原始内容存档于2019 09 24 Matsuda S Ichigotani Y Okuda T Irimura T Nakatsugawa S Hamaguchi M Molecular cloning and characterization of a novel human gene HERNA which encodes a putative RNA helicase Biochimica et Biophysica Acta Jan 2000 1490 1 2 163 9 PMID 10786632 doi 10 1016 S0167 4781 99 00221 3 Hammond SM Dicing and slicing the core machinery of the RNA interference pathway FEBS Letters Oct 2005 579 26 5822 9 PMID 16214139 doi 10 1016 j febslet 2005 08 079 11 0 11 1 Cenik ES Fukunaga R Lu G Dutcher R Wang Y Tanaka Hall TM Zamore PD Phosphate and R2D2 restrict the substrate specificity of Dicer 2 an ATP driven ribonuclease Molecular Cell Apr 2011 42 2 172 84 PMC 3115569 nbsp PMID 21419681 doi 10 1016 j molcel 2011 03 002 取自 https zh wikipedia org w index php title Dicer amp oldid 73593201, 维基百科，wiki，书籍，书籍，图书馆，
