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维基百科

SECIS元件

四月 01, 2023

SECIS元件全稱為硒半胱氨酸插入序列（SElenoCysteine Insertion Sequence），是生物mRNA中一個長約60nt的RNA元件，次級結構為一莖環[1] ，此元件會使核糖體轉譯時不將UGA當作終止密碼子，而將其作為編碼特殊胺基酸硒半胱氨酸的密碼子，因此為編碼硒蛋白的mRNA所需。三域生物皆有SECIS元件[2][3][4][5]，雖皆為莖環結構，但彼此序列差異很大（不過洛基古菌（英语：Lokiarchaeota）的SECIS元件序列和真核生物的較為接近）[6]，細菌的SECIS元件通常緊跟在UGA密碼子之後，真核生物與古菌的SECIS元件則位於mRNA的3'非轉譯區，可使mRNA中多於一個UGA密碼子編碼硒半胱氨酸，另外已知甲烷球菌屬的一種古菌具有位於5'非轉譯區的SECIS元件[7][8]。真核生物的SECIS元件通常有特殊的A：G鹼基配對，可能對其功能相當重要[9]。SECIS元件結合蛋白2（SECIS-binding protein 2，簡稱SBP2）可與SECIS元件結合以促進UGA密碼子編碼硒半胱氨酸[10]，並可避免mRNA被無義介導的mRNA降解（NMD）途徑降解移除[11]。

SECIS元件
一個SECIS元件可能的次級結構，鹼基標示為依循IUPAC規則
识别符
代号 SECIS_1
Alt.代号 SECIS
Rfam RF00031
其他数据
RNA类型 順式作用元件

目前已有數個生物資訊學軟體可用於尋找基因組中的SECIS元件，有助於尋找新的硒蛋白[12]。

參考文獻

  1. ^ Walczak R, Westhof E, Carbon P, Krol A. A novel RNA structural motif in the selenocysteine insertion element of eukaryotic selenoprotein mRNAs. RNA. April 1996, 2 (4): 367–379. PMC 1369379  . PMID 8634917. 
  2. ^ Mix H, Lobanov AV, Gladyshev VN. SECIS elements in the coding regions of selenoprotein transcripts are functional in higher eukaryotes. Nucleic Acids Research. 2007, 35 (2): 414–423. PMC 1802603  . PMID 17169995. doi:10.1093/nar/gkl1060. 
  3. ^ Cassago A, Rodrigues EM, Prieto EL, Gaston KW, Alfonzo JD, Iribar MP, Berry MJ, Cruz AK, Thiemann OH. Identification of Leishmania selenoproteins and SECIS element. Molecular and Biochemical Parasitology. October 2006, 149 (2): 128–134. PMID 16766053. doi:10.1016/j.molbiopara.2006.05.002. 
  4. ^ Mourier T, Pain A, Barrell B, Griffiths-Jones S. A selenocysteine tRNA and SECIS element in Plasmodium falciparum. RNA. February 2005, 11 (2): 119–122. PMC 1370700  . PMID 15659354. doi:10.1261/rna.7185605. 
  5. ^ Kryukov GV, Castellano S, Novoselov SV, Lobanov AV, Zehtab O, Guigó R, Gladyshev VN. Characterization of mammalian selenoproteomes. Science. May 2003, 300 (5624): 1439–1443 [2021-05-23]. PMID 12775843. doi:10.1126/science.1083516. （原始内容于2018-07-23）. 
  6. ^ Mariotti, Marco; Lobanov, Alexei V.; Manta, Bruno; Santesmasses, Didac; Bofill, Andreu; Guigó, Roderic; Gabaldón, Toni; Gladyshev, Vadim N. Lokiarchaeota Marks the Transition between the Archaeal and Eukaryotic Selenocysteine Encoding Systems. Molecular Biology and Evolution. 2016, 33 (9): 2441–2453. ISSN 0737-4038. doi:10.1093/molbev/msw122. 
  7. ^ Wilting R, Schorling S, Persson BC, Böck A. Selenoprotein synthesis in archaea: identification of an mRNA element of Methanococcus jannaschii probably directing selenocysteine insertion. Journal of Molecular Biology. March 1997, 266 (4): 637–641. PMID 9102456. doi:10.1006/jmbi.1996.0812. 
  8. ^ Rother M, Resch A, Wilting R, Böck A. Selenoprotein synthesis in archaea. BioFactors. 2001, 14 (1–4): 75–83. PMID 11568443. doi:10.1002/biof.5520140111. 
  9. ^ JULIA E. FLETCHER, PAUL R. COPELAND, DONNA M. DRISCOLL, ALAIN KROL. The selenocysteine incorporation machinery: Interactions between the SECIS RNA and the SECIS-binding protein SBP2 (PDF). RNA. 2001, 7: 1442-1453 [2021-05-23]. （原始内容 (PDF)于2021-05-23）. 
  10. ^ Donovan J, Copeland PR. Selenocysteine insertion sequence binding protein 2L is implicated as a novel post-transcriptional regulator of selenoprotein expression.. PLoS One. 2012, 7 (4): e35581. PMC 3328465  . PMID 22530054. doi:10.1371/journal.pone.0035581. 
  11. ^ Chellan B, Zhao L, Landeche M, Carmean CM, Dumitrescu AM, Sargis RM. Selenocysteine insertion sequence binding protein 2 (Sbp2) in the sex-specific regulation of selenoprotein gene expression in mouse pancreatic islets.. Sci Rep. 2020, 10 (1): 18568. PMC 7596060  . PMID 33122797. doi:10.1038/s41598-020-75595-4. 
  12. ^ Lambert A, Lescure A, Gautheret D. A survey of metazoan selenocysteine insertion sequences. Biochimie. September 2002, 84 (9): 953–959. PMID 12458087. doi:10.1016/S0300-9084(02)01441-4. 

四月 01, 2023
secis元件, 全稱為硒半胱氨酸插入序列, selenocysteine, insertion, sequence, 是生物mrna中一個長約60nt的rna元件, 次級結構為一莖環, 此元件會使核糖體轉譯時不將uga當作終止密碼子, 而將其作為編碼特殊胺基酸硒半胱氨酸的密碼子, 因此為編碼硒蛋白的mrna所需, 三域生物皆有, 雖皆為莖環結構, 但彼此序列差異很大, 不過洛基古菌, 英语, lokiarchaeota, 的序列和真核生物的較為接近, 細菌的通常緊跟在uga密碼子之後, 真核生物與古菌的則位於mr. SECIS元件全稱為硒半胱氨酸插入序列 SElenoCysteine Insertion Sequence 是生物mRNA中一個長約60nt的RNA元件 次級結構為一莖環 1 此元件會使核糖體轉譯時不將UGA當作終止密碼子 而將其作為編碼特殊胺基酸硒半胱氨酸的密碼子 因此為編碼硒蛋白的mRNA所需 三域生物皆有SECIS元件 2 3 4 5 雖皆為莖環結構 但彼此序列差異很大 不過洛基古菌 英语 Lokiarchaeota 的SECIS元件序列和真核生物的較為接近 6 細菌的SECIS元件通常緊跟在UGA密碼子之後 真核生物與古菌的SECIS元件則位於mRNA的3 非轉譯區 可使mRNA中多於一個UGA密碼子編碼硒半胱氨酸 另外已知甲烷球菌屬的一種古菌具有位於5 非轉譯區的SECIS元件 7 8 真核生物的SECIS元件通常有特殊的A G鹼基配對 可能對其功能相當重要 9 SECIS元件結合蛋白2 SECIS binding protein 2 簡稱SBP2 可與SECIS元件結合以促進UGA密碼子編碼硒半胱氨酸 10 並可避免mRNA被無義介導的mRNA降解 NMD 途徑降解移除 11 SECIS元件一個SECIS元件可能的次級結構 鹼基標示為依循IUPAC規則识别符代号 SECIS 1Alt 代号 SECISRfam RF00031其他数据RNA类型 順式作用元件目前已有數個生物資訊學軟體可用於尋找基因組中的SECIS元件 有助於尋找新的硒蛋白 12 參考文獻 编辑 分子与细胞生物学主题 Walczak R Westhof E Carbon P Krol A A novel RNA structural motif in the selenocysteine insertion element of eukaryotic selenoprotein mRNAs RNA April 1996 2 4 367 379 PMC 1369379 PMID 8634917 Mix H Lobanov AV Gladyshev VN SECIS elements in the coding regions of selenoprotein transcripts are functional in higher eukaryotes Nucleic Acids Research 2007 35 2 414 423 PMC 1802603 PMID 17169995 doi 10 1093 nar gkl1060 Cassago A Rodrigues EM Prieto EL Gaston KW Alfonzo JD Iribar MP Berry MJ Cruz AK Thiemann OH Identification of Leishmania selenoproteins and SECIS element Molecular and Biochemical Parasitology October 2006 149 2 128 134 PMID 16766053 doi 10 1016 j molbiopara 2006 05 002 Mourier T Pain A Barrell B Griffiths Jones S A selenocysteine tRNA and SECIS element in Plasmodium falciparum RNA February 2005 11 2 119 122 PMC 1370700 PMID 15659354 doi 10 1261 rna 7185605 Kryukov GV Castellano S Novoselov SV Lobanov AV Zehtab O Guigo R Gladyshev VN Characterization of mammalian selenoproteomes Science May 2003 300 5624 1439 1443 2021 05 23 PMID 12775843 doi 10 1126 science 1083516 原始内容存档于2018 07 23 Mariotti Marco Lobanov Alexei V Manta Bruno Santesmasses Didac Bofill Andreu Guigo Roderic Gabaldon Toni Gladyshev Vadim N Lokiarchaeota Marks the Transition between the Archaeal and Eukaryotic Selenocysteine Encoding Systems Molecular Biology and Evolution 2016 33 9 2441 2453 ISSN 0737 4038 doi 10 1093 molbev msw122 Wilting R Schorling S Persson BC Bock A Selenoprotein synthesis in archaea identification of an mRNA element of Methanococcus jannaschii probably directing selenocysteine insertion Journal of Molecular Biology March 1997 266 4 637 641 PMID 9102456 doi 10 1006 jmbi 1996 0812 Rother M Resch A Wilting R Bock A Selenoprotein synthesis in archaea BioFactors 2001 14 1 4 75 83 PMID 11568443 doi 10 1002 biof 5520140111 JULIA E FLETCHER PAUL R COPELAND DONNA M DRISCOLL ALAIN KROL The selenocysteine incorporation machinery Interactions between the SECIS RNA and the SECIS binding protein SBP2 PDF RNA 2001 7 1442 1453 2021 05 23 原始内容存档 PDF 于2021 05 23 Donovan J Copeland PR Selenocysteine insertion sequence binding protein 2L is implicated as a novel post transcriptional regulator of selenoprotein expression PLoS One 2012 7 4 e35581 PMC 3328465 PMID 22530054 doi 10 1371 journal pone 0035581 Chellan B Zhao L Landeche M Carmean CM Dumitrescu AM Sargis RM Selenocysteine insertion sequence binding protein 2 Sbp2 in the sex specific regulation of selenoprotein gene expression in mouse pancreatic islets Sci Rep 2020 10 1 18568 PMC 7596060 PMID 33122797 doi 10 1038 s41598 020 75595 4 Lambert A Lescure A Gautheret D A survey of metazoan selenocysteine insertion sequences Biochimie September 2002 84 9 953 959 PMID 12458087 doi 10 1016 S0300 9084 02 01441 4 取自 https zh wikipedia org w index php title SECIS元件 amp oldid 70955370, 维基百科，wiki，书籍，书籍，图书馆，

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