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维基百科

中介體

十一月 09, 2023

中介體（Mediator）舊稱DRIP或TRAP[1][2]，是真核生物細胞核中啟動基因轉錄所需的蛋白質複合體[3][4]，於1990年由美國生物學家罗杰·科恩伯格發現[5][6]。中介體可與RNA聚合酶Ⅱ的C端結構域和轉錄因子結合[7]，結合在基因的強化子和啟動子之間，組成轉錄前起始複合體。RNA轉錄起始後中介體和轉錄因子不會馬上自啟動子脫離，而會維持在該處以繼續結合新的RNA聚合酶Ⅱ，開啟新一輪的轉錄[3]。

中介體與一些週期蛋白依賴性激酶結合
酵母菌的中介體，可見其中MED14（英语：MED14）蛋白結構多變的內在無序結構域

中介體的組成蛋白因物種而異[3]，如釀酒酵母的中介體有21個核心蛋白，哺乳類的中介體則有約30個，許多中介體的蛋白具有內在無序結構域（英语：Intrinsically disordered proteins），為結構較為彈性的區域，可能有助於其改變結構以達成不同功能[8]，除組成的核心蛋白外，中介體還會與一些週期蛋白依賴性激酶（CDK）結合，形成轉錄前起始複合體時與中介體結合的CDK會自其脫落，使RNA聚合酶Ⅱ得以與中介體結合[9]。中介體還會與一個非編碼RNAncRNA-a結合，與其功能有關[10]。

除了調控mRNA（RNA聚合酶Ⅱ）的轉錄外，還有研究顯示酵母菌的中介體參與RNA聚合酶III轉錄tRNA的過程[11][12]。另外中介體可能還可維持中節與端粒的結構[3]，有些中介體的核心蛋白（MED15（英语：MED15）、MED23（英语：MED23）、MED25（英语：MED25））參與訊息傳遞路徑，可能為各種訊息傳遞路徑最終藉由影響中介體的蛋白以調控基因表現[3][13][14]。

參考文獻 编辑

  1. ^ Oda Y, Chalkley RJ, Burlingame AL, Bikle DD. The transcriptional coactivator DRIP/mediator complex is involved in vitamin D receptor function and regulates keratinocyte proliferation and differentiation.. J Invest Dermatol. 2010, 130 (10): 2377–88. PMC 4114506  . PMID 20520624. doi:10.1038/jid.2010.148. 
  2. ^ Ito M, Okano HJ, Darnell RB, Roeder RG. The TRAP100 component of the TRAP/Mediator complex is essential in broad transcriptional events and development.. EMBO J. 2002, 21 (13): 3464–75. PMC 126097  . PMID 12093747. doi:10.1093/emboj/cdf348. 
  3. ^ 3.0 3.1 3.2 3.3 3.4 Allen BL, Taatjes DJ. The Mediator complex: a central integrator of transcription. Nature Reviews Molecular Cell Biology. March 2015, 16 (3): 155–66. PMC 4963239  . PMID 25693131. doi:10.1038/nrm3951. 
  4. ^ Biddick R, Young ET. Yeast mediator and its role in transcriptional regulation. Comptes Rendus Biologies. September 2005, 328 (9): 773–82. PMID 16168358. doi:10.1016/j.crvi.2005.03.004. 
  5. ^ Kelleher RJ, Flanagan PM, Kornberg RD. A novel mediator between activator proteins and the RNA polymerase II transcription apparatus. Cell. June 1990, 61 (7): 1209–15. PMID 2163759. doi:10.1016/0092-8674(90)90685-8. 
  6. ^ Flanagan PM, Kelleher RJ, Sayre MH, Tschochner H, Kornberg RD. A mediator required for activation of RNA polymerase II transcription in vitro. Nature. April 1991, 350 (6317): 436–8. Bibcode:1991Natur.350..436F. PMID 2011193. doi:10.1038/350436a0. 
  7. ^ Björklund S, Gustafsson CM. The yeast Mediator complex and its regulation. Trends in Biochemical Sciences. May 2005, 30 (5): 240–4. PMID 15896741. doi:10.1016/j.tibs.2005.03.008. 
  8. ^ Robinson PJ, Trnka MJ, Pellarin R, Greenberg CH, Bushnell DA, Davis R, Burlingame AL, Sali A, Kornberg RD. Molecular architecture of the yeast Mediator complex. eLife. September 2015, 4: e08719. PMC 4631838  . PMID 26402457. doi:10.7554/eLife.08719. 
  9. ^ Petrenko, N; Jin, Y; Wong, KH; Struhl, K. Mediator Undergoes a Compositional Change during Transcriptional Activation.. Molecular Cell. 3 November 2016, 64 (3): 443–454. PMC 5096951  . PMID 27773675. doi:10.1016/j.molcel.2016.09.015. 
  10. ^ Lai, F; et al. (PDF). Nature. 2013, 494 (7438): 497–501 [2021-05-23]. Bibcode:2013Natur.494..497L. PMC 4109059  . PMID 23417068. doi:10.1038/nature11884. hdl:11858/00-001M-0000-0019-1122-4. （原始内容 (PDF)存档于2017-08-14）. 
  11. ^ Carlsten, JO; Zhu X, López MD, Samuelsson T, Gustafsson CM. Loss of the Mediator subunit Med20 affects transcription of tRNA and other non-coding RNA genes in fission yeast. Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms. February 2016, 1859 (2): 339–347. PMID 26608234. doi:10.1016/j.bbagrm.2015.11.007. 
  12. ^ Uthe H, Vanselow JT, Schlosser A. Proteomic Analysis of the Mediator Complex Interactome in Saccharomyces cerevisiae. Sci Rep. 2017, 7: 43584. Bibcode:2017NatSR...743584U. PMC 5327418  . PMID 28240253. doi:10.1038/srep43584. 
  13. ^ Chen, Rong; Jiang, Hongling; Li, Lin; Zhai, Qingzhe; Qi, Linlin; Zhou, Wenkun; Liu, Xiaoqiang; Li, Hongmei; Zheng, Wenguang; Sun, Jiaqiang; Li, Chuanyou. The Arabidopsis Mediator Subunit MED25 Differentially Regulates Jasmonate and Abscisic Acid Signaling through Interacting with the MYC2 and ABI5 Transcription Factors. The Plant Cell. July 2012, 24 (7): 2898–2916. PMC 3426122  . PMID 22822206. doi:10.1105/tpc.112.098277. 
  14. ^ Sun, Wenjing; Han, Hongyu; Deng, Lei; Sun, Chuanlong; Xu, Yiran; Lin, Lihao; Ren, Panrong; Zhao, Jiuhai; Zhai, Qingzhe; Li, Chuanyou. Mediator Subunit MED25 Physically Interacts with PHYTOCHROME INTERACTING FACTOR4 to Regulate Shade-Induced Hypocotyl Elongation in Tomato. Plant Physiology. November 2020, 184 (3): 1549–1562. PMC 7608172  . PMID 32938743. doi:10.1104/pp.20.00587. 

十一月 09, 2023
中介體, mediator, 舊稱drip或trap, 是真核生物細胞核中啟動基因轉錄所需的蛋白質複合體, 於1990年由美國生物學家罗杰, 科恩伯格發現, 可與rna聚合酶, 的c端結構域和轉錄因子結合, 結合在基因的強化子和啟動子之間, 組成轉錄前起始複合體, rna轉錄起始後和轉錄因子不會馬上自啟動子脫離, 而會維持在該處以繼續結合新的rna聚合酶, 開啟新一輪的轉錄, 與一些週期蛋白依賴性激酶結合酵母菌的, 可見其中med14, 英语, med14, 蛋白結構多變的內在無序結構域的組成蛋白因物種而異, 如釀. 中介體 Mediator 舊稱DRIP或TRAP 1 2 是真核生物細胞核中啟動基因轉錄所需的蛋白質複合體 3 4 於1990年由美國生物學家罗杰 科恩伯格發現 5 6 中介體可與RNA聚合酶 的C端結構域和轉錄因子結合 7 結合在基因的強化子和啟動子之間 組成轉錄前起始複合體 RNA轉錄起始後中介體和轉錄因子不會馬上自啟動子脫離 而會維持在該處以繼續結合新的RNA聚合酶 開啟新一輪的轉錄 3 中介體與一些週期蛋白依賴性激酶結合酵母菌的中介體 可見其中MED14 英语 MED14 蛋白結構多變的內在無序結構域中介體的組成蛋白因物種而異 3 如釀酒酵母的中介體有21個核心蛋白 哺乳類的中介體則有約30個 許多中介體的蛋白具有內在無序結構域 英语 Intrinsically disordered proteins 為結構較為彈性的區域 可能有助於其改變結構以達成不同功能 8 除組成的核心蛋白外 中介體還會與一些週期蛋白依賴性激酶 CDK 結合 形成轉錄前起始複合體時與中介體結合的CDK會自其脫落 使RNA聚合酶 得以與中介體結合 9 中介體還會與一個非編碼RNAncRNA a結合 與其功能有關 10 除了調控mRNA RNA聚合酶 的轉錄外 還有研究顯示酵母菌的中介體參與RNA聚合酶III轉錄tRNA的過程 11 12 另外中介體可能還可維持中節與端粒的結構 3 有些中介體的核心蛋白 MED15 英语 MED15 MED23 英语 MED23 MED25 英语 MED25 參與訊息傳遞路徑 可能為各種訊息傳遞路徑最終藉由影響中介體的蛋白以調控基因表現 3 13 14 參考文獻 编辑 nbsp 分子与细胞生物学主题 Oda Y Chalkley RJ Burlingame AL Bikle DD The transcriptional coactivator DRIP mediator complex is involved in vitamin D receptor function and regulates keratinocyte proliferation and differentiation J Invest Dermatol 2010 130 10 2377 88 PMC 4114506 nbsp PMID 20520624 doi 10 1038 jid 2010 148 Ito M Okano HJ Darnell RB Roeder RG The TRAP100 component of the TRAP Mediator complex is essential in broad transcriptional events and development EMBO J 2002 21 13 3464 75 PMC 126097 nbsp PMID 12093747 doi 10 1093 emboj cdf348 3 0 3 1 3 2 3 3 3 4 Allen BL Taatjes DJ The Mediator complex a central integrator of transcription Nature Reviews Molecular Cell Biology March 2015 16 3 155 66 PMC 4963239 nbsp PMID 25693131 doi 10 1038 nrm3951 Biddick R Young ET Yeast mediator and its role in transcriptional regulation Comptes Rendus Biologies September 2005 328 9 773 82 PMID 16168358 doi 10 1016 j crvi 2005 03 004 Kelleher RJ Flanagan PM Kornberg RD A novel mediator between activator proteins and the RNA polymerase II transcription apparatus Cell June 1990 61 7 1209 15 PMID 2163759 doi 10 1016 0092 8674 90 90685 8 Flanagan PM Kelleher RJ Sayre MH Tschochner H Kornberg RD A mediator required for activation of RNA polymerase II transcription in vitro Nature April 1991 350 6317 436 8 Bibcode 1991Natur 350 436F PMID 2011193 doi 10 1038 350436a0 Bjorklund S Gustafsson CM The yeast Mediator complex and its regulation Trends in Biochemical Sciences May 2005 30 5 240 4 PMID 15896741 doi 10 1016 j tibs 2005 03 008 Robinson PJ Trnka MJ Pellarin R Greenberg CH Bushnell DA Davis R Burlingame AL Sali A Kornberg RD Molecular architecture of the yeast Mediator complex eLife September 2015 4 e08719 PMC 4631838 nbsp PMID 26402457 doi 10 7554 eLife 08719 Petrenko N Jin Y Wong KH Struhl K Mediator Undergoes a Compositional Change during Transcriptional Activation Molecular Cell 3 November 2016 64 3 443 454 PMC 5096951 nbsp PMID 27773675 doi 10 1016 j molcel 2016 09 015 Lai F et al Activating RNAs associate with mediator to enhance chromatin architecture and transcription PDF Nature 2013 494 7438 497 501 2021 05 23 Bibcode 2013Natur 494 497L PMC 4109059 nbsp PMID 23417068 doi 10 1038 nature11884 hdl 11858 00 001M 0000 0019 1122 4 原始内容 PDF 存档于2017 08 14 Carlsten JO Zhu X Lopez MD Samuelsson T Gustafsson CM Loss of the Mediator subunit Med20 affects transcription of tRNA and other non coding RNA genes in fission yeast Biochimica et Biophysica Acta BBA Gene Regulatory Mechanisms February 2016 1859 2 339 347 PMID 26608234 doi 10 1016 j bbagrm 2015 11 007 Uthe H Vanselow JT Schlosser A Proteomic Analysis of the Mediator Complex Interactome in Saccharomyces cerevisiae Sci Rep 2017 7 43584 Bibcode 2017NatSR 743584U PMC 5327418 nbsp PMID 28240253 doi 10 1038 srep43584 Chen Rong Jiang Hongling Li Lin Zhai Qingzhe Qi Linlin Zhou Wenkun Liu Xiaoqiang Li Hongmei Zheng Wenguang Sun Jiaqiang Li Chuanyou The Arabidopsis Mediator Subunit MED25 Differentially Regulates Jasmonate and Abscisic Acid Signaling through Interacting with the MYC2 and ABI5 Transcription Factors The Plant Cell July 2012 24 7 2898 2916 PMC 3426122 nbsp PMID 22822206 doi 10 1105 tpc 112 098277 Sun Wenjing Han Hongyu Deng Lei Sun Chuanlong Xu Yiran Lin Lihao Ren Panrong Zhao Jiuhai Zhai Qingzhe Li Chuanyou Mediator Subunit MED25 Physically Interacts with PHYTOCHROME INTERACTING FACTOR4 to Regulate Shade Induced Hypocotyl Elongation in Tomato Plant Physiology November 2020 184 3 1549 1562 PMC 7608172 nbsp PMID 32938743 doi 10 1104 pp 20 00587 取自 https zh wikipedia org w index php title 中介體 amp oldid 76861521, 维基百科，wiki，书籍，书籍，图书馆，

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