彗星实验（英語：comet assay），又称作单细胞凝胶电泳实验或彗星电泳法（英語：Single Cell Gel Electrophoresis assay，SCGE）是一种在单个真核细胞水平上检测DNA损伤的实验技术。彗星实验最初由Östling和Johansson在1984年发明，此后由 Singh等在1988年改进，此后成为了间的DNA损伤与修复、遗传毒性生物监测的最常用的标准实验技术。It involves the encapsulation of cells in a low-melting-point agarose suspension, lysis of the cells in neutral or alkaline (pH>13) conditions, and electrophoresis of the suspended lysed cells. The term "comet" refers to the pattern of DNA migration through the electrophoresis gel, which often resembles a comet.[1][2]
参考文献编辑
^Tice, R. R. Single Cell Gel/Comet Assay: Guidelines for in vitro and in vivo Genetic Toxicology Testing. Environmental and Molecular Mutagenesis. 2000, 35 (3): 206–21. PMID 10737956. doi:10.1002/(SICI)10982280.
^Nandhakumar S, Parasuraman S, Shanmugam M M, Rao K R, Chand P, Bhat B V. Evaluation of DNA damage using single-cell gel electrophoresis (Comet Assay).
延伸阅读编辑
Dhawan & Anderson (2009): The Comet Assay in Toxicology. doi:10.1039/9781847559746
Avishai, Nanthawan; Rabinowitz, Claudette; Moiseeva, Elisabeth & Rinkevich, Baruch (2002): Genotoxicity of the Kishon River, Israel: the application of an in vitro cellular assay. Mutation Research518(1): 21–37. doi:10.1016/S1383-5718(02)00069-4 (HTML abstract)
Collins, A.R.; Dobson, V.L.; Dusinska, M.; Kennedy, G. & Stetina, R. (1997): The comet assay: what can it really tell us? Mutation Research375(2): 183-193. doi:10.1016/S0027-5107(97)00013-4 (HTML abstract)
Klaude, M.; Eriksson, S.; Nygren, J. & Ahnstrom, G. (1996): The comet assay: mechanisms and technical considerations. Mutation Research363(2): 89-96. doi:10.1016/0921-8777(95)00063-1
McKelvey-Martin, Valerie J.; Ho, Edwin T.; McKeown, Stephanie R.; Johnston, S. Robin; McCarthy, Patsy J.; Rajab, Nor Fasilah & Downes, C. Stephen (1993): Emerging applications of the single cell gel electrophoresis (Comet) assay. I. Management of invasive transitional cell human bladder carcinoma. II. Fluorescent in situ hybridization Comets for the identification of damaged and repaired DNA sequences in individual cells. Mutagenesis13(1): 1-8. PDF fulltext
Olive, P.L.; Wlodek, D. & Banath, J.P. (1991): DNA double-strand breaks measured in individual cells subjected to gel electrophoresis. Cancer Research51(17): 4671-4676. PDF fulltext （页面存档备份，存于互联网档案馆）
Rojas, E.; Lopez, M.C. & Valverde, M. (1999): Single cell gel electrophoresis: methodology and applications. Journal of Chromatography B722(1-2): 225-254. doi:10.1016/S0378-4347(98)00313-2 (HTML abstract)
Singh, N.P.; McCoy, M.T.; Tice, R.R. & Schneider, E.L. (1988): A simple technique for quantitation of low levels of DNA damage in individual cells. Experimental Cell Research175(1): 184-191. doi:10.1016/0014-4827(88)90265-0
Tice, R.R.; Agurell, E.; Anderson, D.; Burlinson, B.; Hartmann, A.; Kobayashi, H.; Miyamae, Y.; Rojas, E.; Ryu, J.-C. & Sasaki, Y.F. (2000): Single Cell Gel/Comet Assay: Guidelines for In Vitro and In Vivo Genetic Toxicology Testing. Environmental and Molecular Mutagenesis35(3): 206-221. doi:10.1002/(SICI)10982280
九月 16, 2023
彗星实验, 英語, comet, assay, 又称作单细胞凝胶电泳实验或彗星电泳法, 英語, single, cell, electrophoresis, assay, scge, 是一种在单个真核细胞水平上检测dna损伤的实验技术, 最初由östling和johansson在1984年发明, 此后由, singh等在1988年改进, 此后成为了间的dna损伤与修复, 遗传毒性生物监测的最常用的标准实验技术, involves, encapsulation, cells, melting, point, agar. 彗星实验 英語 comet assay 又称作单细胞凝胶电泳实验或彗星电泳法 英語 Single Cell Gel Electrophoresis assay SCGE 是一种在单个真核细胞水平上检测DNA损伤的实验技术 彗星实验最初由Ostling和Johansson在1984年发明 此后由 Singh等在1988年改进 此后成为了间的DNA损伤与修复 遗传毒性生物监测的最常用的标准实验技术 It involves the encapsulation of cells in a low melting point agarose suspension lysis of the cells in neutral or alkaline pH gt 13 conditions and electrophoresis of the suspended lysed cells The term comet refers to the pattern of DNA migration through the electrophoresis gel which often resembles a comet 1 2 参考文献 编辑 Tice R R Single Cell Gel Comet Assay Guidelines for in vitro and in vivo Genetic Toxicology Testing Environmental and Molecular Mutagenesis 2000 35 3 206 21 PMID 10737956 doi 10 1002 SICI 10982280 Nandhakumar S Parasuraman S Shanmugam M M Rao K R Chand P Bhat B V Evaluation of DNA damage using single cell gel electrophoresis Comet Assay 延伸阅读 编辑Dhawan amp Anderson 2009 The Comet Assay in Toxicology doi 10 1039 9781847559746 Avishai Nanthawan Rabinowitz Claudette Moiseeva Elisabeth amp Rinkevich Baruch 2002 Genotoxicity of the Kishon River Israel the application of an in vitro cellular assay Mutation Research 518 1 21 37 doi 10 1016 S1383 5718 02 00069 4 HTML abstract Collins A R Dobson V L Dusinska M Kennedy G amp Stetina R 1997 The comet assay what can it really tell us Mutation Research 375 2 183 193 doi 10 1016 S0027 5107 97 00013 4 HTML abstract Klaude M Eriksson S Nygren J amp Ahnstrom G 1996 The comet assay mechanisms and technical considerations Mutation Research 363 2 89 96 doi 10 1016 0921 8777 95 00063 1 McKelvey Martin Valerie J Ho Edwin T McKeown Stephanie R Johnston S Robin McCarthy Patsy J Rajab Nor Fasilah amp Downes C Stephen 1993 Emerging applications of the single cell gel electrophoresis Comet assay I Management of invasive transitional cell human bladder carcinoma II Fluorescent in situ hybridization Comets for the identification of damaged and repaired DNA sequences in individual cells Mutagenesis 13 1 1 8 PDF fulltext Olive P L Wlodek D amp Banath J P 1991 DNA double strand breaks measured in individual cells subjected to gel electrophoresis Cancer Research 51 17 4671 4676 PDF fulltext 页面存档备份 存于互联网档案馆 Rojas E Lopez M C amp Valverde M 1999 Single cell gel electrophoresis methodology and applications Journal of Chromatography B 722 1 2 225 254 doi 10 1016 S0378 4347 98 00313 2 HTML abstract Singh N P McCoy M T Tice R R amp Schneider E L 1988 A simple technique for quantitation of low levels of DNA damage in individual cells Experimental Cell Research 175 1 184 191 doi 10 1016 0014 4827 88 90265 0 Tice R R Agurell E Anderson D Burlinson B Hartmann A Kobayashi H Miyamae Y Rojas E Ryu J C amp Sasaki Y F 2000 Single Cell Gel Comet Assay Guidelines for In Vitro and In Vivo Genetic Toxicology Testing Environmental and Molecular Mutagenesis 35 3 206 221 doi 10 1002 SICI 10982280 取自 https zh wikipedia org w index php title 彗星实验 amp oldid 72359559, 维基百科，wiki，书籍，书籍，图书馆，
