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维基百科

Muse細胞

二月 03, 2024

Muse細胞（Multi-lineage differentiating stress enduring cell，多向分化壓力耐受細胞）是由日本東北大學出澤真理課題組於2010年發現一種內源性、無成瘤性的多能幹細胞[1]。幾乎所有不同器官來源的結締組織（如骨髓、外周血）中都有Muse細胞[2][3][4]。通常Muse細胞可從商業化的間充質細胞中分離到，如人成纖維細胞、骨髓間充質幹細胞，以及脂肪源幹細胞（adipose-derived stem cells）[5][6]。Muse細胞可自發分化或在細胞因子誘導作用下分化爲三個胚層的細胞。Muse細胞的成瘤性較低，注入新的宿主體內也不會形成腫瘤，目前的觀點認爲這是因爲該細胞的端粒酶活性較低[1]。Muse細胞同時被應用於小鼠骨骼肌退變、糖尿病性皮膚潰瘍及急性重症肝炎等模型的修復研究中，研究發現Muse細胞在損傷組織中能夠和宿主的組織順利整合，並且分別向相應胚層的細胞分化[7][8][9]。有研究利用Muse細胞修復腦動脈堵塞後，引起局部缺血的中風小鼠模型，發現Muse細胞修現可以替代丟失的神經元，整合至梗死的皮質組織中，分化為神經元標誌物表達陽性的細胞，並且有較為明顯的功能修復[10]。

培養 编辑

當Muse細胞被放置到含有營養因子和細胞因子的培養基中進行培養時，超過百分之九十的細胞都會進行定向細胞分化。若該培養基含有肝細胞生長因子、成纖維細胞生長因子4和地塞米松的胰島素-轉鐵蛋白-亞硒酸鈉時，百分之九十以上的細胞會分化成肝細胞，並且在α-胎兒蛋白和人類白蛋白表達中呈現陽性反應。在骨或脂肪細胞的培養基中，百分之九十八以上的細胞會分化為骨鈣蛋白或油紅O陽性的細胞[11]。

參見 编辑

參考資料 编辑

  1. ^ 1.0 1.1 Kuroda, Y.; Kitada, M.; Wakao, S.; Nishikawa, K.; Tanimura, Y.; Makinoshima, H.; Goda, M.; Akashi, H.; Inutsuka, A.; Niwa, A.; Shigemoto, T.; Nabeshima, Y.; Nakahata, T.; Nabeshima, Y.-i.; Fujiyoshi, Y.; Dezawa, M. Unique multipotent cells in adult human mesenchymal cell populations. Proceedings of the National Academy of Sciences. 2010, 107 (19): 8639–43. Bibcode:2010PNAS..107.8639K. PMC 2889306  . PMID 20421459. doi:10.1073/pnas.0911647107. 
  2. ^ Wakao, S.; Kitada, M.; Kuroda, Y.; Shigemoto, T.; Matsuse, D.; Akashi, H.; Tanimura, Y.; Tsuchiyama, K.; Kikuchi, T.; Goda, M.; Nakahata, T.; Fujiyoshi, Y.; Dezawa, M. Multilineage-differentiating stress-enduring (Muse) cells are a primary source of induced pluripotent stem cells in human fibroblasts. Proceedings of the National Academy of Sciences. 2011, 108 (24): 9875–80. Bibcode:2011PNAS..108.9875W. PMC 3116385  . PMID 21628574. doi:10.1073/pnas.1100816108. 
  3. ^ Dezawa, Mari. Muse Cells Provide the Pluripotency of Mesenchymal Stem Cells: Direct Contribution of Muse Cells to Tissue Regeneration. Cell Transplantation. 2016, 25 (5): 849–61. PMID 26884346. doi:10.3727/096368916X690881. 
  4. ^ Hori, Emiko; Hayakawa, Yumiko; Hayashi, Tomohide; Hori, Satoshi; Okamoto, Soushi; Shibata, Takashi; Kubo, Michiya; Horie, Yukio; Sasahara, Masakiyo; Kuroda, Satoshi. Mobilization of Pluripotent Multilineage-Differentiating Stress-Enduring Cells in Ischemic Stroke. Journal of Stroke and Cerebrovascular Diseases. 2016, 25 (6): 1473–81. PMID 27019988. doi:10.1016/j.jstrokecerebrovasdis.2015.12.033. 
  5. ^ Kuroda, Yasumasa; Wakao, Shohei; Kitada, Masaaki; Murakami, Toru; Nojima, Makoto; Dezawa, Mari. Isolation, culture and evaluation of multilineage-differentiating stress-enduring (Muse) cells. Nature Protocols. 2013, 8 (7): 1391–415. PMID 23787896. doi:10.1038/nprot.2013.076. 
  6. ^ Heneidi, Saleh; Simerman, Ariel A.; Keller, Erica; Singh, Prapti; Li, Xinmin; Dumesic, Daniel A.; Chazenbalk, Gregorio. Awakened by Cellular Stress: Isolation and Characterization of a Novel Population of Pluripotent Stem Cells Derived from Human Adipose Tissue. PLoS ONE. 2013, 8 (6): e64752. Bibcode:2013PLoSO...864752H. PMC 3673968  . PMID 23755141. doi:10.1371/journal.pone.0064752. 
  7. ^ Simerman, AA; Dumesic, DA; Chazenbalk, GD. Pluripotent muse cells derived from human adipose tissue: a new perspective on regenerative medicine and cell therapy.. Clinical and translational medicine. 2014, 3: 12 [2019-11-22]. PMID 24940477. doi:10.1186/2001-1326-3-12. 
  8. ^ Wakao, S; Akashi, H; Kushida, Y; Dezawa, M. Muse cells, newly found non-tumorigenic pluripotent stem cells, reside in human mesenchymal tissues.. Pathology international. 2014-01, 64 (1): 1–9 [2019-11-22]. PMID 24471964. doi:10.1111/pin.12129. 
  9. ^ Kuroda, Y; Dezawa, M. Mesenchymal stem cells and their subpopulation, pluripotent muse cells, in basic research and regenerative medicine.. Anatomical record (Hoboken, N.J. : 2007). 2014-01, 297 (1): 98–110 [2019-11-22]. PMID 24293378. doi:10.1002/ar.22798. 
  10. ^ Yamauchi, T; Kuroda, Y; Morita, T; Shichinohe, H; Houkin, K; Dezawa, M; Kuroda, S. Therapeutic effects of human multilineage-differentiating stress enduring (MUSE) cell transplantation into infarct brain of mice.. PloS one. 2015, 10 (3): e0116009 [2019-11-22]. PMID 25747577. doi:10.1371/journal.pone.0116009. 
  11. ^ Ogura, Fumitaka; Wakao, Shohei; Kuroda, Yasumasa; Tsuchiyama, Kenichiro; Bagheri, Mozhdeh; Heneidi, Saleh; Chazenbalk, Gregorio; Aiba, Setsuya; Dezawa, Mari. Human Adipose Tissue Possesses a Unique Population of Pluripotent Stem Cells with Nontumorigenic and Low Telomerase Activities: Potential Implications in Regenerative Medicine. Stem Cells and Development. 2014, 23 (7): 717–28. PMID 24256547. doi:10.1089/scd.2013.0473. 

二月 03, 2024
muse細胞, multi, lineage, differentiating, stress, enduring, cell, 多向分化壓力耐受細胞, 是由日本東北大學出澤真理課題組於2010年發現一種內源性, 無成瘤性的多能幹細胞, 幾乎所有不同器官來源的結締組織, 如骨髓, 外周血, 中都有, 通常可從商業化的間充質細胞中分離到, 如人成纖維細胞, 骨髓間充質幹細胞, 以及脂肪源幹細胞, adipose, derived, stem, cells, 可自發分化或在細胞因子誘導作用下分化爲三個胚層的細胞, 的成. Muse細胞 Multi lineage differentiating stress enduring cell 多向分化壓力耐受細胞 是由日本東北大學出澤真理課題組於2010年發現一種內源性 無成瘤性的多能幹細胞 1 幾乎所有不同器官來源的結締組織 如骨髓 外周血 中都有Muse細胞 2 3 4 通常Muse細胞可從商業化的間充質細胞中分離到 如人成纖維細胞 骨髓間充質幹細胞 以及脂肪源幹細胞 adipose derived stem cells 5 6 Muse細胞可自發分化或在細胞因子誘導作用下分化爲三個胚層的細胞 Muse細胞的成瘤性較低 注入新的宿主體內也不會形成腫瘤 目前的觀點認爲這是因爲該細胞的端粒酶活性較低 1 Muse細胞同時被應用於小鼠骨骼肌退變 糖尿病性皮膚潰瘍及急性重症肝炎等模型的修復研究中 研究發現Muse細胞在損傷組織中能夠和宿主的組織順利整合 並且分別向相應胚層的細胞分化 7 8 9 有研究利用Muse細胞修復腦動脈堵塞後 引起局部缺血的中風小鼠模型 發現Muse細胞修現可以替代丟失的神經元 整合至梗死的皮質組織中 分化為神經元標誌物表達陽性的細胞 並且有較為明顯的功能修復 10 培養 编辑當Muse細胞被放置到含有營養因子和細胞因子的培養基中進行培養時 超過百分之九十的細胞都會進行定向細胞分化 若該培養基含有肝細胞生長因子 成纖維細胞生長因子4和地塞米松的胰島素 轉鐵蛋白 亞硒酸鈉時 百分之九十以上的細胞會分化成肝細胞 並且在a 胎兒蛋白和人類白蛋白表達中呈現陽性反應 在骨或脂肪細胞的培養基中 百分之九十八以上的細胞會分化為骨鈣蛋白或油紅O陽性的細胞 11 參見 编辑胚胎幹細胞 間充質 iPS細胞參考資料 编辑 1 0 1 1 Kuroda Y Kitada M Wakao S Nishikawa K Tanimura Y Makinoshima H Goda M Akashi H Inutsuka A Niwa A Shigemoto T Nabeshima Y Nakahata T Nabeshima Y i Fujiyoshi Y Dezawa M Unique multipotent cells in adult human mesenchymal cell populations Proceedings of the National Academy of Sciences 2010 107 19 8639 43 Bibcode 2010PNAS 107 8639K PMC 2889306 nbsp PMID 20421459 doi 10 1073 pnas 0911647107 Wakao S Kitada M Kuroda Y Shigemoto T Matsuse D Akashi H Tanimura Y Tsuchiyama K Kikuchi T Goda M Nakahata T Fujiyoshi Y Dezawa M Multilineage differentiating stress enduring Muse cells are a primary source of induced pluripotent stem cells in human fibroblasts Proceedings of the National Academy of Sciences 2011 108 24 9875 80 Bibcode 2011PNAS 108 9875W PMC 3116385 nbsp PMID 21628574 doi 10 1073 pnas 1100816108 Dezawa Mari Muse Cells Provide the Pluripotency of Mesenchymal Stem Cells Direct Contribution of Muse Cells to Tissue Regeneration Cell Transplantation 2016 25 5 849 61 PMID 26884346 doi 10 3727 096368916X690881 Hori Emiko Hayakawa Yumiko Hayashi Tomohide Hori Satoshi Okamoto Soushi Shibata Takashi Kubo Michiya Horie Yukio Sasahara Masakiyo Kuroda Satoshi Mobilization of Pluripotent Multilineage Differentiating Stress Enduring Cells in Ischemic Stroke Journal of Stroke and Cerebrovascular Diseases 2016 25 6 1473 81 PMID 27019988 doi 10 1016 j jstrokecerebrovasdis 2015 12 033 Kuroda Yasumasa Wakao Shohei Kitada Masaaki Murakami Toru Nojima Makoto Dezawa Mari Isolation culture and evaluation of multilineage differentiating stress enduring Muse cells Nature Protocols 2013 8 7 1391 415 PMID 23787896 doi 10 1038 nprot 2013 076 Heneidi Saleh Simerman Ariel A Keller Erica Singh Prapti Li Xinmin Dumesic Daniel A Chazenbalk Gregorio Awakened by Cellular Stress Isolation and Characterization of a Novel Population of Pluripotent Stem Cells Derived from Human Adipose Tissue PLoS ONE 2013 8 6 e64752 Bibcode 2013PLoSO 864752H PMC 3673968 nbsp PMID 23755141 doi 10 1371 journal pone 0064752 Simerman AA Dumesic DA Chazenbalk GD Pluripotent muse cells derived from human adipose tissue a new perspective on regenerative medicine and cell therapy Clinical and translational medicine 2014 3 12 2019 11 22 PMID 24940477 doi 10 1186 2001 1326 3 12 Wakao S Akashi H Kushida Y Dezawa M Muse cells newly found non tumorigenic pluripotent stem cells reside in human mesenchymal tissues Pathology international 2014 01 64 1 1 9 2019 11 22 PMID 24471964 doi 10 1111 pin 12129 Kuroda Y Dezawa M Mesenchymal stem cells and their subpopulation pluripotent muse cells in basic research and regenerative medicine Anatomical record Hoboken N J 2007 2014 01 297 1 98 110 2019 11 22 PMID 24293378 doi 10 1002 ar 22798 Yamauchi T Kuroda Y Morita T Shichinohe H Houkin K Dezawa M Kuroda S Therapeutic effects of human multilineage differentiating stress enduring MUSE cell transplantation into infarct brain of mice PloS one 2015 10 3 e0116009 2019 11 22 PMID 25747577 doi 10 1371 journal pone 0116009 Ogura Fumitaka Wakao Shohei Kuroda Yasumasa Tsuchiyama Kenichiro Bagheri Mozhdeh Heneidi Saleh Chazenbalk Gregorio Aiba Setsuya Dezawa Mari Human Adipose Tissue Possesses a Unique Population of Pluripotent Stem Cells with Nontumorigenic and Low Telomerase Activities Potential Implications in Regenerative Medicine Stem Cells and Development 2014 23 7 717 28 PMID 24256547 doi 10 1089 scd 2013 0473 取自 https zh wikipedia org w index php title Muse細胞 amp oldid 74166105, 维基百科，wiki，书籍，书籍，图书馆，

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